Plant Tissue Culture

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1. Introduction

Plant tissue culture (PTC) is a set of techniques for the aseptic culture of cells, tissues, organs and their components under defined physical and chemical conditions in vitro and controlled environment (Fig. 50.1). PTC technology also explores conditions that promote cell division and genetic re-programming in in vitro conditions and it is considered an important tool in both basic and applied studies, as well as in commercial application (1).Fig. 50.1.

A. Callus from Catharanthus roseus. B. Suspension culture from C. roseus. C. Regeneration of plantlets from C. roseus callus. D. Tumors from C. roseus. E. Protoplasts from C. roseus. F. Micropropagation of Agave tequilana. G. Hairy roots from C. roseus. H. Somatic embryogenesis of Coffea canephora. I. Root culture from C. roseus. Pictures A–E, G–I from the authors' laboratories. Picture F from the laboratory of Dr. Manuel Robert all of them at Centro de Investigación Cientifica de Yucatán