Protocol

PCR Detection of Microbial Pathogens

Volume 943 of the series Methods in Molecular Biology pp 135-147

Date:

Utilization of Multiple Real-Time PCR Assays for the Diagnosis of Bordetella spp. in Clinical Specimens

  • Kathleen M. TattiAffiliated withDivision of Bacterial Diseases, Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Meningitis and Vaccine Preventable Diseases Branch Email author 
  • , Maria Lucia TondellaAffiliated withDivision of Bacterial Diseases, Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Meningitis and Vaccine Preventable Diseases Branch

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Abstract

Bordetella pertussis causes an upper respiratory infection in infants, adolescents, and adults. Diagnosis of pertussis, a vaccine-preventable disease, can be difficult, but recent implementation of real-time PCR assays in laboratories has hastened the ability of clinicians to make an accurate diagnosis. In this paper we describe the method of nasopharyngeal specimen collection, extraction of DNA, and real-time PCR assays that will allow the detection and identification of Bordetella spp. in clinical specimens.