Protocol

G Protein-Coupled Receptors in Drug Discovery

Volume 552 of the series Methods in Molecular Biology pp 199-211

Date:

BacMam: Versatile Gene Delivery Technology for GPCR Assays

  • Elizabeth A. DavenportAffiliated withMolecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D
  • , Parvathi NuthulagantiAffiliated withMolecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D
  • , Robert S. AmesAffiliated withMolecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D

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Summary

BacMam viruses are modified baculoviruses that contain mammalian expression cassettes for viral gene delivery and transient expression in mammalian cells. They are easily, inexpensively, and rapidly generated and provide a versatile solution for G protein-coupled receptor (GPCR) cell-based assay development. Using BacMam technology, target gene expression levels are easily controlled and simultaneous delivery of multiple genes is possible, for example, coexpression of a receptor and a G protein or a reporter gene. BacMam viruses are compatible with the GPCR cell-based assay formats typically used in high-throughput screening and provide an unparalleled level of experimental flexibility that is simply not possible when using stable recombinant cell lines.

Key words

BacMam Modified baculovirus Transient expression Viral transduction G protein-coupled receptors GPCR Cell-based assay