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Molecular Profiling pp 157–178Cite as

Laser Capture Microdissection: ArcturusXT Infrared Capture and UV Cutting Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 823))

Abstract

Laser capture microdissection (LCM) is a technique that allows the precise procurement of enriched cell populations from a heterogeneous tissue under direct microscopic visualization. LCM can be used to harvest the cells of interest directly or can be used to isolate specific cells by ablating the unwanted cells, resulting in histologically enriched cell populations. The fundamental components of laser microdissection technology are (a) visualization of the cells of interest via microscopy, (b) transfer of laser energy to a thermolabile polymer with either the formation of a polymer–cell composite (capture method) or transfer of laser energy via an ultraviolet laser to photovolatize a region of tissue (cutting method), and (c) removal of cells of interest from the heterogeneous tissue section. Laser energy supplied by LCM instruments can be infrared (810 nm) or ultraviolet (355 nm). Infrared lasers melt thermolabile polymers for cell capture, whereas ultraviolet lasers ablate cells for either removal of unwanted cells or excision of a defined area of cells. LCM technology is applicable to an array of applications including mass spectrometry, DNA genotyping and loss-of-heterozygosity analysis, RNA transcript profiling, cDNA library generation, proteomics discovery, and signal kinase pathway profiling. This chapter describes the unique features of the ArcturusXT laser capture microdissection instrument, which incorporates both infrared capture and ultraviolet cutting technology in one instrument, using a proteomic downstream assay as a model.

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Author information

Authors and Affiliations

  1. Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA

    Rosa I. Gallagher, Lance A. Liotta & Virginia Espina

  2. Applied Biosystems/Life Technologies Corporation, Foster City, CA, USA

    Steven R. Blakely

Authors
  1. Rosa I. Gallagher
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  2. Steven R. Blakely
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  3. Lance A. Liotta
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  4. Virginia Espina
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Corresponding author

Correspondence to Rosa I. Gallagher .

Editor information

Editors and Affiliations

  1. Center for Applied Proteomics and, George Mason University, University Blvd. 10900, Manassas, 20110, Virginia, USA

    Virginia Espina

  2. Center for Applied Proteomics &, George Mason University, University Blvd. 10900, Manassas, 20110, Virginia, USA

    Lance A. Liotta

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Gallagher, R.I., Blakely, S.R., Liotta, L.A., Espina, V. (2012). Laser Capture Microdissection: ArcturusXT Infrared Capture and UV Cutting Methods. In: Espina, V., Liotta, L. (eds) Molecular Profiling. Methods in Molecular Biology, vol 823. Humana Press. https://doi.org/10.1007/978-1-60327-216-2_11

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  • DOI: https://doi.org/10.1007/978-1-60327-216-2_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-215-5

  • Online ISBN: 978-1-60327-216-2

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