Protocol

Germline Stem Cells

Volume 450 of the series Methods in Molecular Biology™ pp 181-192

Deriving Mouse Spermatogonial Stem Cell Lines

  • Ilaria FalciatoriAffiliated withDepartment of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center
  • , Kate Lillard-WetherellAffiliated withDepartment of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center
  • , Zhuoru WuAffiliated withDepartment of Pharmacology, University of Texas Southwestern Medical Center
  • , F. Kent HamraAffiliated withDepartment of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center
  • , David L. GarbersAffiliated withDepartment of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center

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Summary

In adult males, spermatogonial stem cells function to replenish developing gametes that are continuously released from the testes as mature spermatozoa. Because of their potential importance to research, medicine, industry, and conservation, numerous attempts have been made in the past to cultivate sperma-togonial stem cells in vitro. However, only recently have culture methods been established that effectively promote the proliferation of mammalian spermatogonial stem cells in vitro. We describe a simple and reproducible protocol for the derivation and maintenance of mouse spermatogonial stem cell lines that proliferate for long periods of time in culture.

Keywords

Germ cells germline stem cells mouse spermatogonia spermatogonial stem cells spermatogonial stem cell culture SSC testis