Protocol

Mesenchymal Stem Cells

Volume 449 of the series Methods in Molecular Biology™ pp 69-79

Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens

  • Severine G. DuboisAffiliated withPennington Biomedical Research Center
  • , Elizabeth Z. FloydAffiliated withPennington Biomedical Research Center
  • , Sanjin ZvonicAffiliated withPennington Biomedical Research Center
  • , Gail KilroyAffiliated withPennington Biomedical Research Center
  • , Xiying WuAffiliated withPennington Biomedical Research Center
  • , Stacy CarlingAffiliated withPennington Biomedical Research Center
  • , Yuan Di C. HalvorsenAffiliated withCuragen Corp.
  • , Eric RavussinAffiliated withPennington Biomedical Research Center
  • , Jeffrey M. GimbleAffiliated withPennington Biomedical Research Center

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Abstract

Adipose tissue has proven to serve as an abundant, accessible, and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe a detailed method for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens and a small scale procedure suitable for processing adipose tissue biopsy specimens of < 0.5 g. Although we have focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.

Keywords

Adipose-derived stem cells (ASCs) biopsy expansion human isolation lipoaspirate mesenchymal stem cells (MSCs) stromal vascular fraction (SVF)