Protocol

2D PAGE: Sample Preparation and Fractionation

Volume 424 of the series Methods in Molecular Biology™ pp 365-372

Isolation of Phosphoproteins

  • Lawrence G. PuenteAffiliated withThe Ottawa Hospital
  • , Lynn A. MegeneyAffiliated withThe Ottawa Hospital

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Summary

Phosphorylation is one of the most abundant post-translational modifications on protein and one that frequently has functional biological consequences. For this reason, screening protein samples for phosphorylations has become an important tool in biochemical research. Affinity purification by immunological or chemical reagents can be used to isolate phosphoproteins from other cellular materials.

Key Words

Affinity purification immunoprecipitation protein phosphorylation