Protocol

Transgenesis Techniques

Volume 561 of the series Methods in Molecular Biology pp 245-263

Date:

Site-Specific Recombinases for Manipulation of the Mouse Genome

  • Marie-Christine BirlingAffiliated withInstitut Clinique de la Souris – Mouse Clinical Institute (ICS-MCI)
  • , Françoise GofflotAffiliated withInstitut Clinique de la Souris – Mouse Clinical Institute (ICS-MCI)
  • , Xavier WarotAffiliated withInstitut Clinique de la Souris – Mouse Clinical Institute (ICS-MCI)

* Final gross prices may vary according to local VAT.

Get Access

Summary

Site-specific recombination systems are widespread and popular tools for all scientists interested in manipulating the mouse genome. In this chapter, we focus on the use of site-specific recombinases (SSR) to unravel the function of genes of the mouse. In the first part, we review the most commonly used SSR, Cre and Flp, as well as the newly developed systems such as Dre and PhiC31, and we present the inducible SSR systems. As experience has shown that these systems are not as straightforward as expected, particular attention is paid to facts and artefacts associated with their production and applications to study the mouse genome. In the next part of this chapter, we illustrate new applications of SSRs that allow engineering of the mouse genome with more and more precision, including the FLEX and the RMCE strategies. We conclude and suggest a workflow procedure that can be followed when using SSR to create your mouse model of interest. Together, these strategies and procedures provide the basis for a wide variety of studies that will ultimately lead to the analysis of the function of a gene at the cellular level in the mouse.

Key words

Site-specific recombinases Conditional knockout Cre Flp PhiC31 Inducible systems FLEX RMCE Mouse model engineering