Protocol

Apoptosis

Volume 559 of the series Methods in Molecular Biology pp 343-356

Date:

Detection of Cell Death in Drosophila

  • Kimberly McCallAffiliated withDepartment of Biology, Boston University
  • , Jeanne S. PetersonAffiliated withDepartment of Biology, Boston University
  • , Tracy L. PritchettAffiliated withDepartment of Biology, Boston University

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Summary

Drosophila is a powerful model system for the identification of cell death genes and understanding the role of cell death in development. In this chapter, we describe three methods typically used for the detection of cell death in Drosophila. The TUNEL and acridine orange methods are used to detect dead or dying cells in a variety of tissues. We focus on methods for the embryo and the ovary, but these techniques can be used on other tissues as well. The third method is the detection of genetic interactions by expressing cell death genes in the Drosophila eye.

Key words

Drosophila Apoptosis Cell death Embryo Ovary