Protocol

Drosophila

Volume 420 of the series Methods in Molecular Biology pp 175-195

Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis

  • Johannes BischofAffiliated withInstitute of Molecular Biology, University of Zürich
  • , Konrad BaslerAffiliated withInstitute of Molecular Biology, University of Zürich

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Abstract

The site-specific recombinase FLP is used in Drosophila to precisely manipulate the genome, in particular, to eliminate gene function by mitotic recombination and to activate transgenes in discrete populations of cells. These approaches are already part of the standard tool kit for studying gene function. The number of applications for the FLP recombinase has increased over the years and further members of the large family of site-specific recombinases are being added to the arsenal of fly geneticists, most recently, the φC31 integrase. This chapter will introduce these recombinases and describe how such instruments are utilized to accurately manipulate the Drosophila genome.

Key Words

Drosophila melanogaster Cre/loxP FLP/FRT φC31 integrase mitotic recombination mosaics recombinases transgenesis