Protocol

Innate Immunity

Volume 415 of the series Methods in Molecular Biology™ pp 119-126

Intravital Two-Photon Imaging of Natural Killer Cells and Dendritic Cells in Lymph Nodes

  • Susanna CelliAffiliated withDépartement d’Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir
  • , Béatrice BreartAffiliated withDépartement d’Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir
  • , Philippe BoussoAffiliated withDépartement d’Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir

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Summary

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.

Key Words

NK cells dendritic cells lymph node intravital imaging two-photon microscopy