Protocol

Innate Immunity

Volume 415 of the series Methods in Molecular Biology™ pp 403-427

Models of Caenorhabditis elegans Infection by Bacterial and Fungal Pathogens

  • Jennifer R. PowellAffiliated withDepartment of Molecular Biology, Massachusetts General HospitalDepartment of Genetics, Harvard Medical School
  • , Frederick M. AusubelAffiliated withDepartment of Molecular Biology, Massachusetts General HospitalDepartment of Genetics, Harvard Medical School

* Final gross prices may vary according to local VAT.

Get Access

Summary

The nematode Caenorhabditis elegans is a simple model host for studying the relationship between the animal innate immune system and a variety of bacterial and fungal pathogens. Extensive genetic and molecular tools are available in C. elegans, facilitating an in-depth analysis of host defense factors and pathogen virulence factors. Many of these factors are conserved in insects and mammals, indicating the relevance of the nematode model to the vertebrate innate immune response. Here, we describe pathogen assays for a selection of the most commonly studied bacterial and fungal pathogens using the C. elegans model system.

Keywords

Caenorhabditis elegans innate immunity pathogenesis Cryptococcus neoformans Drechmeria coniospora Enterococcus faecalis Microbacterium nematophilum Pseudomonas aeruginosa Salmonella enterica Serratia marcescens Staphylococcus aureus Yersinia pestis Yersinia pseudotuberculosis