Protocol

Innate Immunity

Volume 415 of the series Methods in Molecular Biology™ pp 395-402

Investigating the Involvement of Host Factors Involved in Intracellular Pathogen Infection by RNAi in Drosophila Cells

  • Hervé AgaisseAffiliated withSection of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine

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Summary

Intracellular pathogens represent a serious threat to human health. Although the biology and the virulence factors involved in intracellular bacterial infection are relatively well documented, little is known about the host factors involved in the infection process. This situation is mainly due to the difficulty of conducting extensive genetic analyses in the targeted host cells and points to the need for developing genetic systems to model the infection process. Here, we describe a method (amenable to high-throughput analysis) that allows for the study of host factors involved in intracellular pathogen infection by using dsRNA-mediated gene expression knockdown [RNA interference (RNAi)] in Drosophila cell lines.

Key Words

Host/pathogen interaction intracellular pathogen innate immunity Drosophila Listeria RNAi immunofluorescence microscopy high-throughput screening