Innate Immunity

Volume 415 of the series Methods in Molecular Biology™ pp 379-394

Drosophila Immunity

Methods for Monitoring the Activity of Toll and Imd Signaling Pathways
  • Yves RomeoAffiliated withCentre de Génétique Moléculaire du CNRS, Gif-sur-Yvette
  • , Bruno LemaitreAffiliated withCentre de Génétique Moléculaire du CNRS, Gif-sur-Yvette

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Invertebrates lack an adaptive immune system and rely on innate immunity to resist pathogens. The response of Drosophila melanogaster to bacterial and fungal infections involves two signaling pathways, Toll and Imd, both of which activate members of the nuclear factor (NF)-κB family of transcription factors, leading to antimicrobial peptide (AMP) gene expression. In this chapter, we present the current methods used in our laboratory to monitor the activity of both signaling pathways.

Key Words

Drosophila immunity Toll and Imd signaling pathways antimicrobial peptides reporter genes RT-qPCR survival analyses