Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads
- Charles H. LinAffiliated withIllumina, Inc.
- , Joanne M. YeakleyAffiliated withIllumina, Inc.
- , Timothy K. McDanielAffiliated withIllumina, Inc.
- , Richard ShenAffiliated withIllumina, Inc.
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Recent breakthroughs in multiplexed SNP (single nucleotide polymorphism) genotyping technology have enabled global mapping of the relationships between genetic variation and disease. Discoveries made by such whole-genome association studies often spur further interest in surveying more focused subsets of SNPs for validation or research purposes. Here we describe a new SNP genotyping platform that is flexible in assay content and multiplexing (up to 384 analytes), and can serve medium- to high-throughput applications. The Illumina BeadXpress platform supports the GoldenGate Genotyping Assay on digitally inscribed VeraCode microbeads to allow streamlined workflow, rapid detection, unparalleled data reproducibility and consistency. Thus, it is a highly valuable tool for biomarker research and validation, pharmaceutical development, as well as the development of molecular diagnostic tests.
Key WordsHigh-throughput genotyping biomarker validation SNP randomly assembled microarrays allele-specific extension and ligation universal capture oligonucleotides universal arrays
- Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads
- Book Title
- DNA and RNA Profiling in Human Blood
- Book Subtitle
- Methods and Protocols
- Book Part
- Part I
- pp 129-142
- Print ISBN
- Online ISBN
- Series Title
- METHODS IN MOLECULAR BIOLOGY™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press, a part of Springer Science+Business Media, LLC
- Additional Links
- High-throughput genotyping
- biomarker validation
- randomly assembled microarrays
- allele-specific extension and ligation
- universal capture oligonucleotides
- universal arrays
- Industry Sectors
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