Protocol

Biological Microarrays

Volume 671 of the series Methods in Molecular Biology pp 107-116

Date:

Lithographically Defined Two- and Three-Dimensional Tissue Microarrays

  • Esther W. GomezAffiliated withDepartments of Chemical Engineering and Molecular Biology, Princeton University
  • , Celeste M. NelsonAffiliated withDepartment of Chemical Engineering, Princeton University

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Abstract

Traditional methods to study normal and pathological development of tissues have been limited by ­difficulties in controlling experimental conditions and quantifying biological processes of interest. Here we describe methods to create microarrays of engineered tissues that enable controlled and quantitative investigations. Using soft lithography-based techniques, extracellular matrix proteins can be microcontact printed or micromolded to make two- and three-dimensional micropatterned scaffolds. The ultimate form and resulting properties of the tissue construct are dictated by the geometry of the patterned extracellular matrix components. This chapter describes elastomeric stamp fabrication, microcontact printing and micromolding of extracellular matrix proteins, cell culture in micropatterned substrata, and quantitative immunofluorescence analysis of micropatterned tissues.

Key words

Tissue engineering Microfabrication Organotypic culture Epithelial