Methods in Molecular Biology Volume 411, 2007, pp 81-98

Detection of GFP During Nervous System Development in Drosophila melanogaster

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Using the vital marker GFP and its spectral variants, it is possible to visualize multiple proteins in individual cells and thereby monitor embryonic development on a cellular and molecular level. In the following chapter we describe how to prepare Drosophila embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.