Methods in Molecular Biology Volume 411, 2007, pp 47-80

Fluorescent Proteins for Cell Biology

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Through the use of exogenous labels, such as antibodies and synthetic fluorophores, experimenters have been able to readily observe the localization of proteins and organelles within a cell by fluorescence microscopy. The discovery and application of fluorescent proteins spanning a large wavelength range have revolutionized these studies. This chapter attempts to introduce the vast array of these molecules, discuss their characteristics, and assess the advantages and disadvantages that each displays for use in imaging.