Methods in Molecular Biology Volume 411, 2007, pp 1-11

Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues

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Abstract

This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.