Protocol

Cancer Gene Profiling

Volume 576 of the series Methods in Molecular Biology pp 293-326

Date:

Cancer Gene Profiling in Prostate Cancer

  • Adam FoyeAffiliated withDepartments of Medicine and Molecular Genetics and Microbiology, Duke Institute for Genome Science and Policy, Duke University
  • , Phillip G. FebboAffiliated withDepartments of Medicine and Molecular Genetics and Microbiology, Duke Institute for Genome Science and Policy, Duke University

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Summary

Gene profiling and expression analysis using microarrays have made a significant impact on our biological understanding of prostate cancer. The procedures for generating high-quality expression data from prostate cancer cell lines and tumors are not trivial. However, during the past 9 years, methods by which to process samples for gene profiling have been developed. In this chapter, techniques to process prostate cancer specimens either en bloc (macrodissection) or using laser capture microdissection are presented in detail along with extensive technical notes. Although we focus on prostate cancer and discuss the specific methods utilized in our lab, the processes discussed are generalizable to other tumors and amenable to the substitution of alternative instruments and/or commercially available kits.

Key words

Prostate cancer Expression analysis Laser capture microdissection En bloc analysis