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Yeast Functional Genomics and Proteomics

Volume 548 of the series Methods in Molecular Biology pp 101-114

Date:

A Genomic Approach to Yeast Chronological Aging

  • Christopher R. BurtnerAffiliated withDepartment of Pathology, University of Washington
  • , Christopher J. MurakamiAffiliated withDepartment of Pathology, University of Washington
  • , Matt KaeberleinAffiliated withDepartment of Pathology, University of Washington Email author 

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Summary

Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.

Key words

Longevity Aging Chronological life span, Yeast Bioscreen Stationary phase