DNA Microarrays for Biomedical Research

Volume 529 of the series Methods in Molecular Biology pp 279-295


Chromatin Immunoprecipitation Using Microarrays

  • Mickaël Durand-DubiefAffiliated withKarolinska Institute /NOVUM
  • , Karl EkwallAffiliated withUniversity College Södertörn

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Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein–DNA interactions that occur in vivo. This genome-wide analysis of protein–DNA association is carried out in several steps including chemical cross-linking, cell lysis, DNA fragmentation and immunoaffinity purification that allow the identification of DNA interactions and provide a powerful tool for genome-wide investigations. Immunoprecipitated DNA fragments associated with the desired protein are amplified, labelled and hybridized to DNA microarrays to detect enriched signals compared to a labelled reference sample.

Key words

Chromatin immunoprecipitation arrays affymetrix eurogentec ChIP ChIP-chip