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Meiosis

Volume 557 of the series Methods in Molecular Biology pp 77-97

Date:

Analysis of Meiotic Recombination in Caenorhabditis elegans

  • Kenneth J. HillersAffiliated withBiological Sciences, California Polytechnic State University
  • , Anne M. VilleneuveAffiliated withDepartment of Developmental Biology, Stanford University School of Medicine

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Abstract

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

Key words

crossing over PCR recombination chiasmata snip-SNP bivalent univalent