Abstract
Efficient manipulation of Hedgehog (HH)/GLI signaling activity is crucial to the analysis of molecular events underlying HH/GLI-regulated cell fate determination and tumor growth. In this article, we describe the use of retroviral expression systems as a valuable tool to activate or repress Hh-pathway activity in a broad spectrum of mammalian cells—including human cells—either by forced expression of the major Hedgehog-effectors GLI1 and GLI2 or by expression of the short-hairpin RNAs-targeting GLI mRNAs. We focus on two distinct retroviral systems that allow efficient and sustainable expression of GLI proteins in primary cells and cell lines of human origin: (i) a Moloney Murine Leukemia Virus-based and (ii) an HIV-derived lentivirus expression system, which allows transduction of both dividing and quiescent cells.
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© 2007 Humana Press Inc., Totowa, NJ
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Kasper, M., Regl, G., Eichberger, T., Frischauf, AM., Aberger, F. (2007). Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems. In: Horabin, J.I. (eds) Hedgehog Signaling Protocols. Methods Inmolecular Biology™, vol 397. Humana Press. https://doi.org/10.1007/978-1-59745-516-9_6
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DOI: https://doi.org/10.1007/978-1-59745-516-9_6
Publisher Name: Humana Press
Print ISBN: 978-1-58829-692-4
Online ISBN: 978-1-59745-516-9
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