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Comparative Genomics

Volume 395 of the series Methods in Molecular Biology™ pp 503-525

Prediction of Structural Noncoding RNAs With RNAz

  • Stefan WashietlAffiliated withDepartment of Theoretical Chemistry, University of Vienna

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Summary

The function of many noncoding RNAs (ncRNAs) depend on a defined secondary structure. RNAz detects evolutionarily conserved and thermodynamically stable RNA secondary structures in multiple sequence alignments and, thus, efficiently filters for candidate ncRNAs. In this chapter, we provide a step-by-step guide on how to use RNAz. Starting with basic concepts, we also cover advanced analysis techniques and, as an example for a large scale application, demonstrate a complete screen of the Saccharomyces cerevisiae genome.

Key Words

Noncoding RNA gene finding conserved RNA secondary structure RNA structure prediction