Protocol

Molecular Motors

Volume 392 of the series Methods in Molecular Biology™ pp 1-22

Equilibrium Binding of Proteins to F-Actin

  • Joseph M. ChalovichAffiliated withDepartment of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University

* Final gross prices may vary according to local VAT.

Get Access

Abstract

This chapter reviews some of the many available methods for measuring the binding of myosin and other proteins to actin. Binding to actin has special considerations because actin is a long lattice and the binding site of many of its binding partners consists of multiple actin protomers. The analysis of binding to a lattice cannot be done by standard methods such as a Scatchard plot. Rational methods of analysis are described.

Key Words

Myosin S1 actin G-actin F-actin cooperativity kinetics binding binding analysis equilibrium association constant parking problem ATPase rate equations sedimentation ATP