Protocol

Plant Hormones

Volume 495 of the series Methods in Molecular Biology pp 53-60

Date:

Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry

  • Masanori OkamotoAffiliated withGrowth Regulation Research Group, RIKEN Plant Science Center
  • , Atsushi HanadaAffiliated withGrowth Regulation Research Group, RIKEN Plant Science Center
  • , Yuji KamiyaAffiliated withGrowth Regulation Research Group, RIKEN Plant Science Center
  • , Shinjiro YamaguchiAffiliated withGrowth Regulation Research Group, RIKEN Plant Science Center
  • , Eiji NambaraAffiliated withGrowth Regulation Research Group, RIKEN Plant Science Center

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Abstract

Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

Key words

Abscisic acid gibberellins gas chromatography–mass spectrometry (GC-MS) internal standard derivatization