Methods in Molecular Biology Volume 495, 2009, pp 53-60
Date: 20 Oct 2008

Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry

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Abstract

Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.