Protocol

Baculovirus and Insect Cell Expression Protocols

Volume 388 of the series Methods in Molecular Biology™ pp 95-114

Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

  • James A. FornwaldAffiliated withDepartment of Gene Expression and Protein Biochemistry, GlaxoSmithKline
  • , Quinn LuAffiliated withDepartment of Biological Reagents and Assay Development, GlaxoSmithKline
  • , Dayuan WangAffiliated withDepartment of Biological Reagents and Assay Development, GlaxoSmithKline
  • , Robert S. AmesAffiliated withDepartment of Biological Reagents and Assay Development, GlaxoSmithKline

* Final gross prices may vary according to local VAT.

Get Access

Abstract

BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. The BacMam system combines the advantages of viral transient expression, ease in generation, and a wide cell tropism. It enables rapid, facile, and flexible gene over-expression experiments to be performed in a variety of mammalian cell lines. Conversion of baculovirus vectors to BacMam vectors involves replacement of the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.

Key Words

BacMam modified baculovirus transient expression viral transduction gene over-expression coexpression GPCR FACS