Baculovirus and Insect Cell Expression Protocols

Volume 388 of the series Methods in Molecular Biology™ pp 247-266

Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors

  • Sue H. KadwellAffiliated withDepartment of Biological Reagents and Assay Development, GlaxoSmithKline
  • , Philip I. HardwickeAffiliated withDepartment of Biological Reagents and Assay Development, GlaxoSmithKline, Medicines Research Centre

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Wave Bioreactors are relatively new to the field of protein production from insect cells infected with recombinant baculoviruses. Various sizes are available to support expression needs from small to large scale. They offer many advantages over stirred tank and airlift reactors, including simple operation, ease of setup and clean-up, and minimal utility requirements. The design consists of a platform rocker onto which presterilized and assembled bags are placed. Once inflated, the bags are filled with medium and cells. Filtered air flow maintains the volumetric shape of the bag and provides head space for gas exchange. A “wave” motion is created by the rocking and angle settings of the platform. Monitoring can be as simple or as detailed as the user requires. Excellent insect cell growth and production of proteins from many classes can be achieved with these units.

Key Words

Wave bioreactor “cellbag” or “wavebag” Sf-9 cells Trichoplusia ni cells recombinant baculovirus mass spectrometer cellbag integrity testing