Protocol

Serial Analysis of Gene Expression (SAGE)

Volume 387 of the series Methods in Molecular Biology™ pp 81-93

DeepSAGE

Higher Sensitivity and Multiplexing of Samples Using a Simpler Experimental Protocol
  • Kåre Lehmann NielsenAffiliated withDepartment of Life Sciences, University of Aalborg

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Summary

Combining serial analysis of gene expression (SAGE) with pyrophosphatase-based ultra-high-throughput DNA sequencing provides increased sensitivity and cost-effective gene expression profiling. The combined techniques obviate the formation and cloning of concatemers and the tedious picking and preparation of sequence templates from bacterial clones that are necessary with SAGE alone. Furthermore, multiplexing of samples or replicates of analysis is included in the experimental design.

Key Words

Pyrophosphatase DNA sequencing ditag sequencing high throughput deep sampling.