Protocol

Type 2 Diabetes

Volume 560 of the series Methods in Molecular Biology pp 239-261

Date:

Gene Expression Analysis in Diabetes Research

  • Peter WhiteAffiliated withDepartment of Genetics and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine
  • , Klaus H. KaestnerAffiliated withDepartment of Genetics and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine Email author 

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Summary

Global gene expression profiling through the use of microarray technology is among the most powerful molecular biological techniques available to diabetes researchers today. In this chapter, we outline how to appropriately perform a microarray experiment using pancreatic islets or total pancreas, based upon over a decade of experience in our laboratory. Through the utilization of careful experimental designs, large numbers of biological replicates, production of high-quality starting material, optimized protocols for hybridization, and sophisticated tools for data processing and statistical analysis, the full potential of high-quality expression profiling can be realized.

Key words

Microarray RNA extraction Gene expression profiling Diabetes Islet, β-Cell