Protocol

Immunological Tolerance

Volume 380 of the series Methods in Molecular Biology™ pp 355-363

Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node

  • Susanna CelliAffiliated withDépartment d’Immunologie, Institut Pasteur
  • , Philippe BoussoAffiliated withDépartment d’Immunologie, Institut Pasteur

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Abstract

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.

Key Words

T-cell activation tolerance lymph node intravital imaging two-photon microscopy