Protocol

Hepatitis C

Volume 510 of the series Methods in Molecular Biology™ pp 415-426

Analysis of HCV-Specific T Cells by Flow Cytometry

  • Masaaki ShiinaAffiliated withImmunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseases, National Institutes of Health
  • , Barbara RehermannAffiliated withImmunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseases, National Institutes of Health

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Abstract

Flow cytometry has become an essential research tool because of the increase in the number of its {applications.} The development of an increasing number of monoclonal antibodies (mAbs) and fluorochromes, and of instruments capable of multicolor detection, allows the acquisition of a large amount of phenotypic and functional information in a single assay. In addition, flow-cytometry techniques have overcome critical problems of conventional assays, such as the use of radioactive reagents to assess proliferation and cytotoxicity of virus-specific T cells. Here, we provide both an overview of available techniques as well as standard protocols that have proven valuable in the assessment of HCV-specific T-cell responses.

Key Words

Flow cytometry FACS hepatitis C virus proliferation cytokines cytotoxic T cells