Protocol

Hepatitis C

Volume 510 of the series Methods in Molecular Biology™ pp 403-413

Determination of HCV-Specific T-Cell Activity

  • Joo Chun YoonAffiliated withImmunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseases, National Institutes of Health
  • , Barbara RehermannAffiliated withImmunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseases, National Institutes of Health

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Abstract

The magnitude and breadth of T-cell responses against HCV are associated with the outcome of HCV infection. Parameters of HCV-specific T-cell responses that are frequently assessed in clinical immunological studies include proliferation of T cells in response to HCV antigens, frequency of HCV-specific T cells secreting cytokines, and changes in antigen specificity during the course of HCV infection. Common techniques for assessing these parameters such as 3H-thymidine incorporation assay, cytokine ELISpot assay, and strategies for epitope mapping and identification of minimal optimal epitopes are outlined, and detailed protocols are described.

Key Words

Hepatitis C virus T cell proliferation assay ELISpot assay epitope mapping