Protocol

Glycovirology Protocols

Volume 379 of the series Methods in Molecular Biology pp 51-68

The C Type Lectins DC-SIGN and L-SIGN

Receptors for Viral Glycoproteins
  • Pierre-Yves LozachAffiliated withLaboratoire de Pathogénie Virale Moléculaire, Institut PasteurUMR de Virologie Moléculaire et Structurale, CNRS 2472-INRA 1157
  • , Laura BurleighAffiliated withLaboratoire de Pathogénie Virale Moléculaire, Institut Pasteur
  • , Isabelle StaropoliAffiliated withLaboratoire de Pathogénie Virale Moléculaire, Institut Pasteur
  • , Ali AmaraAffiliated withLaboratoire de Pathogénie Virale Moléculaire, Institut Pasteur

* Final gross prices may vary according to local VAT.

Get Access

Abstract

DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and transfer viral infections to other target cells (trans-infection). In this chapter, we will give an overview of protocols used to produce soluble viral glycoproteins at high levels and to study the molecular basis of viruses/DC-SIGN and L-SIGN binding and internalization. We will also describe techniques to investigate the molecular mechanisms by which DC-SIGN or L-SIGN spread viral infections.

Key Words

DC-SIGN L-SIGN dendritic cells endothelial cells viruses envelope glycoproteins endocytosis viral entry infection viral transmission