Protocol

Avian Influenza Virus

Volume 436 of the series Methods in Molecular Biology™ pp 19-26

Type A Influenza Virus Detection and Quantitation by Real-Time RT-PCR

  • Erica SpackmanAffiliated withSoutheast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service
  • , David L. SuarezAffiliated withSoutheast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service

* Final gross prices may vary according to local VAT.

Get Access

Summary

Real-time RT-PCR (rRT-PCR) is a relatively new technology that has been used for avian influenza (AI) virus detection since the early 2000s for routine surveillance, during outbreaks, and for research. Some of the advantages of rRT-PCR are high sensitivity, high specificity, rapid time-to-result, scalability, cost, and quantitative nature. Furthermore, rRT-PCR can be used with numerous sample types, is less expensive than virus isolation in chicken embryos, and since infectious virus is inactivated early during processing, biosafety and biosecurity are also easier to maintain. This chapter will provide an overview of the USDA-validated rRT-PCR procedure for the detection of type A influenza.

Keywords

real-time RT-PCR hydrolysis probe RNA avian influenza virus detection virus quantitation