Protocol

Cell Fusion

Volume 475 of the series Methods in Molecular Biology™ pp 3-20

Yeast Mating

A Model System for Studying Cell and Nuclear Fusion
  • Casey A. YdenbergAffiliated withDepartment of Molecular Biology, Princeton University
  • , Mark D. RoseAffiliated withDepartment of Molecular Biology, Princeton University

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Summary

Haploid yeast cells mate to form a zygote, whose progeny are diploid cells. A fundamentally sexual event, related to fertilization, yeast mating nevertheless exhibits cytological properties that appear similar to somatic cell fusion. A large collection of mutations that lead to defects in various stages of mating, including cell fusion, has allowed a detailed dissection of the overall pathway. Recent advances in imaging methods, together with powerful methods of genetic analysis, make yeast mating a superb platform for investigation of cell fusion. An understanding of yeast cell fusion will provide insight into fundamental mechanisms of cell signaling, cell polarization, and membrane fusion.

Key Words

Conjugation mating Saccharomyces cerevisiae pheromone cell polarity karyogamy