Methods in Molecular Biology™ Volume 468, 2008, pp 99-110

Assaying β-Catenin/TCF Transcription with β-Catenin/TCF Transcription-Based Reporter Constructs

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Abstract

Transcription-based reporters have been instrumental in characterizing the Wnt/β-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/β-catenin reporter system, β-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.