Protocol

Adipose Tissue Protocols

Volume 456 of the series Methods in Molecular Biology™ pp 39-54

Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass

  • Wei ShenAffiliated withObesity Research Center, St. Luke's-Roosevelt Hospital and Institute of Human Nutrition, Columbia University College of Physicians and Surgeons
  • , Jun ChenAffiliated withImage Reading Lab, Obesity Research Center, St. Luke's-Roosevelt Hospital and Institute of Human Nutrition, Columbia University College of Physicians and Surgeons

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Summary

In vivo adipose tissue quantification is an important tool to characterize phenotypes of obesity, especially in the human. The amount and distribution of adipose tissue is associated with many of the adverse consequences of obesity. Recent studies suggest that adipose tissue is not a single homogeneous compartment. Regional adipose tissue depots vary in biological functions and individual adipose tissue compartments have stronger associations with metabolic conditions than does total adipose tissue mass. Currently there is intense and increasing interest in regional adipose tissue compartments. Computed tomography and magnetic resonance imaging often are used to quantify adipose tissue volumes or cross-sectional adipose tissue areas. Other modalities, including dual-energy absorpti-ometry and magnetic resonance spectroscopy, provide whole-body or regional fat measures instead of adipose tissue mass quantification.

Key words

Magnetic resonance imaging computed tomography adipose tissue visceral adipose tissue subcutaneous adipose tissue intermuscular adipose tissue body composition abdominal obesity