Volume 518 of the series Methods in Molecular Biology pp 113-122


Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

  • Bryan G. AllenAffiliated withDepartment of Biochemistry, University of Iowa
  • , Daniel L. WeeksAffiliated withDepartment of Biochemistry, University of Iowa

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Bacteriophage φC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the φC31 integrase approach to make transgenic Xenopus laevis embryos.

Key words

Xenopus φC31 integrase transgenesis fluorescence