Protocol

Autophagosome and Phagosome

Volume 445 of the series Methods in Molecular Biology™ pp 439-449

The Mycobacterium tuberculosis Phagosome

  • Esteban A. RobertsAffiliated withDepartment of Molecular Genetics and Microbiology, University of New Mexico School of Medicine
  • , Vojo DereticAffiliated withHealth Sciences Center, Department Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center

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Summary

Tuberculosis is currently the most devastating human bacterial disease, causing millions of deaths annually and infecting an overwhelming percentage of the global population. Its success as a scourge lies in the ability of Mycobacterium tuberculosis to prevent normal phagolysosome biogenesis, essential to the destruction of invading microorganisms, inside macrophages. Recent work has identified host GTPases involved in the block of normal phagolysosome biogenesis during mycobacterial infection and has provided a set of methods, in particular efficient macrophage transfection, which will prove essential in examining the role of host effectors in this process.

Key Words

M. tuberculosis phagosome Rabs maturation Rab conversion