Protocol

Neural Stem Cells

Volume 438 of the series Methods in Molecular Biology™ pp 103-121

Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property

  • Seung U. KimAffiliated withDivision of Neurology, UBC Hospital, University of British ColumbiaBrain Disease Research Center, Ajou University School of Medicine
  • , Atsushi NagaiAffiliated withDivision of Neurology, UBC Hospital, University of British Columbia
  • , Eiji NakagawaAffiliated withDivision of Neurology, UBC Hospital, University of British Columbia
  • , Hyun B. ChoiAffiliated withDivision of Neurology, UBC Hospital, University of British Columbia
  • , Jung H. BangAffiliated withDivision of Neurology, UBC Hospital, University of British ColumbiaBrain Disease Research Center, Ajou University School of Medicine
  • , Hong J. LeeAffiliated withDivision of Neurology, UBC Hospital, University of British ColumbiaBrain Disease Research Center, Ajou University School of Medicine
  • , Myung A. LeeAffiliated withBrain Disease Research Center, Ajou University School of Medicine
  • , Yong B. LeeAffiliated withBrain Disease Research Center, Ajou University School of Medicine
  • , In H. ParkAffiliated withBrain Disease Research Center, Ajou University School of Medicine

* Final gross prices may vary according to local VAT.

Get Access

Summary

We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human neural stem cell lines (HB1.F3) was found to express nestin and other specific markers for human neural stem cells, giving rise to three fundamental cell types of the CNS: neurons, astrocytes, and oligodendrocytes. After transplantation into the brain of mouse model of stroke, implanted human neural stem cells were observed to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells.

Key Words

Cell line differentiation glia human immortalization neural stem cells neurons retroviral vector v-myc