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Osteoporosis

Volume 455 of the series Methods In Molecular Biology™ pp 203-235

Quantitative Trait Loci Mapping

  • Dong-Hai XiongAffiliated withOsteoporosis Research Center, Creighton University
  • , Jian-Feng LiuAffiliated withDepartments of Orthopedic Surgery and Basic Medical Sciences, University of Missouri-Kansas City
  • , Yan-Fang GuoAffiliated withThe Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Molecular Genetic, School of Life Science and Technology, Xi'an Jiaotong University
  • , Yan GuoAffiliated withThe Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Molecular Genetic, School of Life Science and Technology, Xi'an Jiaotong University
  • , Tie-Lin YangAffiliated withThe Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Molecular Genetic, School of Life Science and Technology, Xi'an Jiaotong University
  • , Hui JiangAffiliated withLaboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University
  • , Yuan ChenAffiliated withLaboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University
  • , Fang YangAffiliated withLaboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University
  • , Robert R ReckerAffiliated withOsteoporosis Research Center, Creighton University
    • , Hong-Wen DengAffiliated withDepartments of Orthopedic Surgery and Basic Medical Sciences, University of Missouri-Kansas City

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Abstract

This chapter presents current methods for mapping quantitative trait loci (QTLs) in natural populations especially in humans. We discussed the experimental designs for QTL mapping, traditional methods adopted such as linkage mapping approaches and methods for linkage disequilibrium (LD) mapping. Multiple traits and interaction analysis are also outlined. The application of modern genomic approaches, which mainly exploit the microarray technology, into QTL mapping was detailed. The latter are very recent protocols and are less developed than linkage and association methods at present. The main focus of this chapter is technical issues although statistical issues are also covered to certain extent. Finally, we summarize the limitations of the current QTL approaches and discuss the solutions to certain problems.

Keywords

QTL linkage association linkage disequilibrium microarrays eQTL whole genome association.