Protocol

Plant Virology Protocols

Volume 451 of the series Methods in Molecular Biology™ pp 555-562

Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses

  • Zarir E. VaghchhipawalaAffiliated withPlant Biology Division, The Samuel Roberts Noble Foundation
  • , Kirankumar S. MysoreAffiliated withPlant Biology Division, The Samuel Roberts Noble Foundation

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Abstract

Plant-virus interaction studies, for long, plagued by asynchronous/failed infections, have improved since the usage of Agrobacterium as a delivery agent for viral genomes. Popularly known as “agroinoculation,” this method has revolutionized plant virology studies, leading to identification of viruses as casual agents of disease, viral genome mutagenesis and recombination analyses, and virus-induced gene silencing (VIGS) applications. We present here a brief overview of the recent applications of this method and a detailed protocol for agroinoculation and VIGS used in our laboratory.

Keywords

Agrobacterium Agroinoculation Agroinfiltration Agrodrench VIGS Tobacco rattle virus (TRV) vector