Protocol

Stem Cells in Regenerative Medicine

Volume 482 of the series Methods in Molecular Biology pp 407-418

Reprogramming of Liver to Pancreas

  • Shifaan ThowfeequAffiliated withCentre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath
  • , Wan-Chun LiAffiliated withCentre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath
  • , Jonathan M.W. SlackAffiliated withCentre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath
  • , David ToshAffiliated withCentre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath

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Abstract

Islet grafts have demonstrated that patients with diabetes would benefit greatly by β-cell therapy. However, the paucity of available islets for transplantation as well as the immunological barriers faced in allogeneic transplantation represent a tremendous barrier to regenerative approaches to the treatment of diabetes. Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into β-cells by the ectopic expression of critical β-cell transcription factors.

Key words

Two-step collagenase liver perfusion hepatocyte culture pancreatic transcription factors Pdx1 adenovirus transdifferentiation immunofluorescence