Reprogramming of Liver to Pancreas
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Islet grafts have demonstrated that patients with diabetes would benefit greatly by β-cell therapy. However, the paucity of available islets for transplantation as well as the immunological barriers faced in allogeneic transplantation represent a tremendous barrier to regenerative approaches to the treatment of diabetes. Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into β-cells by the ectopic expression of critical β-cell transcription factors.
- Li, W.-C., Yu, W.-Y., Quinlan, J. M., Burke, Z. D., and Tosh, D. (2005) Molecular basis of transdifferentiation. Journal of Cellular and Molecular Medicine 9, 569–582. CrossRef
- Slack, J. M. W. (1995) The developmental biology of the pancreas. Development 121, 1569–1580.
- Michalopoulos, G. K. (1990) Liver regeneration: molecular mechanisms of growth control. FASEB J. 14, 176–187.
- Thomas, C. E., Ehrhardt, A., and Kay, M. A. (2003) Progress and problems with the use of viral vectors for gene therapy. Nature Reviews Genetics 4, 346–358. CrossRef
- McConnell, M. J., and Imperiale, M. J. (2004) Biology of adenovirus and its use as a vector for gene therapy. Human Gene Therapy 15, 1022–1033. CrossRef
- Tosh, D., Alberti, K. G. M. M., and Agius, L. (1988) Glucagon regulation of gluconeogenesis and ketogenesis in periportal and perivenous rat hepatocytes. Biochemical Journal 256, 197–204.
- Tollefson, A. E., Hermiston, T. W., and Wold, W. S. M. (1998) Preparation and titration of CsCl-banded adenovirus stock, in Adenovirus Methods and Protocols, Humana, Totowa, NJ.
- Reprogramming of Liver to Pancreas
- Book Title
- Stem Cells in Regenerative Medicine
- Book Part
- Part VII
- pp 407-418
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Two-step collagenase liver perfusion
- hepatocyte culture
- pancreatic transcription factors
- Industry Sectors
- Editor Affiliations
- 2. University of Toronto
- Author Affiliations
- 3. Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath, UK
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