Protocol

Stem Cells in Regenerative Medicine

Volume 482 of the series Methods in Molecular Biology pp 269-279

Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)

  • Rahul SarugaserAffiliated withInstitute of Biomaterials and Biomedical Engineering, University of Toronto
  • , Jane EnnisAffiliated withInstitute of Biomaterials and Biomedical Engineering, University of Toronto
  • , William L. StanfordAffiliated withInstitute of Biomaterials and Biomedical Engineering, University of Toronto
  • , John E. DaviesAffiliated withInstitute of Biomaterials and Biomedical Engineering, University of Toronto

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Abstract

Current sources of mesenchymal cells, including bone marrow, fat and muscle, all require invasive procurement procedures, and provide relatively low frequencies of progenitors. Here, we describe the non-invasive isolation, and characterization, of a rich source of mesenchymal progenitor cells, which we call human umbilical cord perivascular cells (HUCPVCs). HUCPVCs show a similar immunological phenotype to bone marrow-derived mesenchymal stromal cells (BM-MSCs), since they are non-alloreactive, exhibit immunosuppression, and significantly reduce lymphocyte activation, in vitro. They present a non-hematopoietic myofibroblastic mesenchymal phenotype (CD45-, CD34-, CD105+, CD73+, CD90+, CD44+, CD106+, 3G5+, CD146+); with a 1:300 frequency at harvest, a short-doubling time, and a clonogenic frequency of >1:3 in culture. Furthermore, in addition to robust quinti-potential differentiation capacity in vitro, HUCPVCs have been shown to contribute to both musculo-skeletal and dermal wound healing in vivo.

Key words

Mesenchymal progenitor cell mesenchymal stem cell umbilical cord