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Stem Cells in Regenerative Medicine

Volume 482 of the series Methods in Molecular Biology pp 171-183

Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord

  • Hynek WichterleAffiliated withDepartments of Pathology, Neurology, and Neurobiology and Behavior, Columbia University Medical Center
  • , Mirza PeljtoAffiliated withDepartments of Pathology, Neurology, and Neurobiology and Behavior, Columbia University Medical Center
  • , Stephane NedelecAffiliated withDepartments of Pathology, Neurology, and Neurobiology and Behavior, Columbia University Medical Center

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Abstract

A growing number of specific cell types have been successfully derived from embryonic stem cells (ES cells), including a variety of neural cells. In vitro generated cells need to be extensively characterized to establish functional equivalency with their in vivo counterparts. The ultimate test for the ability of ES cell-derived neurons to functionally integrate into neural networks is transplantation into the developing central nervous system, a challenging technique limited by the poor accessibility of mammalian embryos. Here we describe xenotransplantation of mouse embryonic stem cell-derived motor neurons into the developing chick neural tube as an alternative for testing the ability of in vitro generated neurons to survive, integrate, extend axons, and form appropriate synaptic contacts with functionally relevant targets in vivo. Similar methods can be adapted to study functionality of other mammalian cells, including derivatives of human ES cells.

Key words

Embryonic stem cells motor neurons xenotransplantation chick neural tube spinal cord