Protocol

Mouse Cell Culture

Volume 633 of the series Methods in Molecular Biology pp 91-99

Date:

Isolation and Culture of Embryonic Pancreas and Liver

  • Zoë D. BurkeAffiliated withDepartment of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath
  • , Wan-Chun LiAffiliated withDepartment of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath
  • , Jonathan M.W. SlackAffiliated withDepartment of Biology and Biochemistry, Centre for Regenerative Medicine, University of BathStem Cell Institute, University of Minnesota
  • , David ToshAffiliated withDepartment of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath

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Abstract

Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as providing models for developmental disorders. Here we describe a system for the in vitro culture of the dorsal pancreatic buds and liver buds from mouse embryos. The tissues are dissected from day 9.0 or 11.5 mouse embryos. The tissues are placed on fibronectin-coated coverslips in serum-containing medium and allowed to attach. Over the next few days, the buds grow as flattened structures which are thin enough to allow the use of wholemount immunostaining methods.

Key words

Pancreas liver embryo culture