Protocol

High-Resolution Imaging of Cellular Proteins

Volume 1474 of the series Methods in Molecular Biology pp 171-185

Date:

Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging

  • Gordon B. SmithAffiliated withDepartment of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience
  • , David FitzpatrickAffiliated withDepartment of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience Email author 

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Abstract

Two-photon (2P) imaging has proven to be a powerful tool for investigating neural structure and function both in brain slices and in intact systems. In vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applicable to a range of species, including mice, and are routinely used in ferrets and tree shrews to provide large-scale labeling of cortical volumes and high-quality imaging data.

Key words

Two-photon In vivo Microscopy Imaging Viral expression Genetically encoded calcium sensor