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Enhancer RNAs pp 11–18Cite as

Knockdown of Nuclear-Located Enhancer RNAs and Long ncRNAs Using Locked Nucleic Acid GapmeRs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1468))

Abstract

The human genome is widely transcribed outside of protein-coding genes, producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs. Functional studies to determine the role of individual genes are challenging with noncoding RNAs appearing to be more difficult to knockdown than mRNAs. One factor that may have hindered progress is that the majority of noncoding RNAs are thought to be located within the nucleus, where the efficiency of traditional RNA interference techniques is debatable. Here we present an alternative RNA interference technique utilizing Locked Nucleic Acids, which is able to efficiently knockdown noncoding RNAs irrespective of intracellular location.

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Author information

Authors and Affiliations

  1. Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK

    Benoit T. Roux, Mark A. Lindsay & James A. Heward Ph.D.

  2. Centre for Haemato-Oncology, Barts Cancer Institute, Charterhouse Square, London, EC1M 6BQ, UK

    James A. Heward Ph.D.

Authors
  1. Benoit T. Roux
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  2. Mark A. Lindsay
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  3. James A. Heward Ph.D.
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Corresponding author

Correspondence to James A. Heward Ph.D. .

Editor information

Editors and Affiliations

  1. Max Planck Institute Molecular Genetics, Berlin, Germany

    Ulf Andersson Ørom

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Roux, B.T., Lindsay, M.A., Heward, J.A. (2017). Knockdown of Nuclear-Located Enhancer RNAs and Long ncRNAs Using Locked Nucleic Acid GapmeRs. In: Ørom, U. (eds) Enhancer RNAs. Methods in Molecular Biology, vol 1468. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4035-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-4035-6_2

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4033-2

  • Online ISBN: 978-1-4939-4035-6

  • eBook Packages: Springer Protocols

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